Figure 4.

Role of PvrA in gene regulation during infection. Wild type PA14 and the ΔpvrA mutant were grown in LB to an OD₆₀₀ of 1.0. A portion of the bacteria were subjected to RNA isolation. The remaining bacteria were washed and resuspended in PBS. Mice were infected with the bacteria intranasally. The bacteria were isolated from BALF 6 hpi. The mRNA levels of indicated genes were determined by real time PCR with rpsL (encoding the 16S rRNA) as the internal control. (A). Relative mRNA levels of pvrA of PA14 in BALF and LB medium. (B). Relative mRNA levels of plcH, fadD1, fadD6, PA0508, aprA, glcB, maeB in BALF or LB. *P < 0.05; **P < 0.01; ***P < 0.001 by Student's t-test. Data represent the mean ± standard deviation from three samples.