Figure 6.

Growth analyses and genes expression of PA14 and the ΔpvrA mutant on palmitic acid, glycerine and choline. Same amount of wild type PA14 and the ΔpvrA mutants were inoculated in FA-M9 (A), Gly-M9 (B) or Cho-M9 (C). The bacterial growth was monitored by measuring OD₅₄₀. (D). PA14, the ΔpvrA mutant and the complemented strain were grown in Gly-M9 or FA-M9 for 10 h. The intracellular acetyl-CoA concentration was determined and normalized to the corresponding total protein concentration. (E). PA14 and the ΔpvrA mutant were grown in FA-M9, Gly-M9 or Cho-M9 for 10 h. The relative mRNA levels of fadD1, fadD6, PA0508, plcH, glcB were determined by real time PCR. *P < 0.05; **P < 0.01; ***P < 0.001 by Student's t-test. Data represent the mean ± standard deviation from three samples.