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. 2020 May 14;48(11):5967–5985. doi: 10.1093/nar/gkaa377

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Palmitoyl-coenzyme A enhances the binding of PvrA to the target DNA fragment. The binding of wild type PvrA (A) or the mutated PvrA (R136A) (B) to the fadD1 promoter was examined by EMSA. The fadD1 promoter fragment was incubated with increasing concentrations of PvrA or the mutated PvrA (R136A) with or without the indicated concentrations of palmitoyl-coenzyme A. The DNA bands were visualized by EB staining. The free probes are indicated by arrowheads and the super shifts are indicated by arrows.