Figure 6.

API5 functions in 4E-SE containing mRNA export. (A) RT-qPCR analysis of the subcellular levels of 4E-SE-containing mRNAs following API5 depletion and reconstitution. ‘C’ is the cytosolic fraction and ‘N’ is the nucleus fraction. (B) Protein levels were monitored by western blot analysis for the protein products of the representative 4E-SE-containing genes c-MYC and cyclin D1 following API5 depletion and reconstitution. (C) Western blot analysis showing the c-MYC and cyclin D1 levels following FGF2 depletion. (D) Western blot analysis showing the c-MYC and cyclin D1 levels after treatment with lentivirus expressing the API5-segment 2 peptide. Numbers below the western blots indicate the expression of proteins as measured by fold change.