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. 2020 May 12;48(11):6265–6279. doi: 10.1093/nar/gkaa352

Figure 1.

Figure 1.

RPS28A and RPS28B are important for PB assembly. (A) Log-phase wild-type S.cerevisiae BY4741 (WT), rps28aΔ and rps28bΔ strains were transformed with pRB001 expressing both Pab1-GFP (SG marker) and Edc3-mCh (PB marker) and examined for the presence of PB foci. (B) Quantification of A; average number of PBs per cell. Data generated from 3 biological replicates with mean ± s.d. shown. An ANOVA with Dunnetts post-hoc test was used to assess significance. (C) As in A, except cells were subject to 10 min glucose deprivation stress. (D) Quantification of C; average number of PBs per cell. Data generated from 3 biological replicates with mean ± s.d shown. An ANOVA with Dunnetts post-hoc test was used to assess significance. (E) rps28aΔ and rps28bΔ strains are not defective in global translation repression (polysome reduction indicated by arrowheads). Log-phase WT, rps28aΔ and rps28bΔ strains growing under normal growth conditions and under 10 min of glucose deprivation stress were subject to polysome analysis. Data is representative of observations from three biological replicates.