Figure 2.

RPS28B 3′UTR is important for PB assembly. (A) WT, rps28aΔ and rps28bΔ strains were transformed with empty vector, pRS315 or pRS315 expressing RPS28B + 3′UTR and assessed for the presence of PB foci. (B) Quantitation of data in panel A. Data generated from 3 biological replicates with mean ± s.d shown. An ANOVA with Tukey's post-hoc test was used to asses significance. (C) Western analysis of above strains to assess total Rps28 protein levels; all images shown are from the same gel and exposure, with vertical lines indicating lanes spliced together. (D) Quantification of data in (C). (E) RT-PCR analysis of RPS28B mRNA levels.