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. 2020 May 12;48(11):6265–6279. doi: 10.1093/nar/gkaa352

Figure 3.

Figure 3.

The distal stem loop-containing region of the RPS28B 3′UTR, and Edc3 binding is important for PB formation. (A) Schematic of RPS28B 3′UTR truncations generated. (B) Log-phase rps28bΔ strains were transformed with plasmids harboring different RPS28B 3′UTR truncations and pRB001 expressing both Pab1-GFP (SG marker) and Edc3-mCh (PB marker) and assessed for their effects on PB assembly by fluorescence microscopy. (C) Quantitation of data in panel B. Data generated from 3 biological replicates with mean ± s.d shown. An ANOVA with Dunnetts post-hoc test was used to assess significance. (D, E) Log-phase rps28bΔ strain was transformed with WT RPS28B + 3'UTR, RPS28B ORF-RPS28B 3′UTR with MS2 stem-loops, Edc3-MS2 or a combination of the latter two; Edc3-mCh (PB marker) was also transformed in all strains to visualize the presence of PB foci. (F) Quantification of E; average number of PBs per cell. Data generated from 3 biological replicates with mean ± s.d shown. An ANOVA with Tukey's post-hoc test was used to asses significance.