Figure 5.

The RPS28B 3′UTR alone is not sufficient to rescue PBs in the rps28bΔ strain. (A) Schematic of chimeric constructs used. (B) Log-phase rps28bΔ strains were transformed with chimeric constructs and pRB001 and assessed for presence of PB foci. (C) Data generated from three biological replicates with mean ± s.d. shown. An ANOVA with Dunnetts post-hoc test was used to assess significance. (D) RT-qPCR using RPS28B 3′UTR-specific probe(s) was used to assess chimeric mRNA levels. An ANOVA with Dunnetts post-hoc test was used to assess significance.