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. 2020 May 12;48(11):6265–6279. doi: 10.1093/nar/gkaa352

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Rps28–Edc3 interaction is critical for PB assembly and may occur on polysomes. (A) Schematic of Edc3, indicating Rps28 binding domain. (B) Log-phase edc3Δ RP840 strains transformed with Edc3-mCherry/Edc3ΔRB-mCherry were assessed for Edc3-mCherry PB foci by microscopy. (C) Quantification of data in (B); data was analyzed via a two-tailed student's T-test. (D) Log-phase WT cells transformed with Edc3-GFP were subject to polysome analysis. (E) RT-qPCR was used to assess RPS28B and GAPDH mRNA levels in the polysome fractions corresponding to the polysome profile shown in panel (D). Data generated from three biological replicates with mean ± s.d. shown. (F) Western analysis was used to assess the presence of Edc3-GFP in the polysome fractions corresponding to the polysome profile shown in panel (D). (G) Quantitation of data in panel (F). Data generated from three biological replicates with mean ± s.d. shown.