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. 2020 May 12;48(11):6280–6293. doi: 10.1093/nar/gkaa354

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — PFDs interact with LSM8. (A) BiFC assays in N. benthamiana leaves. PFDs fused to YFP^N were co-expressed with LSM8 fused to YFP^C. Fluorescence from the reconstituted YFP was detected by confocal microscopy from leaf discs three days after infiltration (upper row). YFP^N-HSFA1a, which does not interact with LSM8, was used as negative control. Insets show fluorescence from a representative nucleus. Bright field images are shown in the bottom row. Scale bars: 75 μm. (B) Co-immunoprecipitation assays showing the interaction between PFDs fused to YFP and LSM8-HA in N. benthamiana. Total proteins were immunoprecipitated with anti-GFP antibody-coated paramagnetic beads from extracts of infiltrated N. benthamiana leaves. Proteins were detected with anti-GFP and anti-HA antibodies. (C) Co-immunoprecipitation assays showing the interaction between PFD4 and PFD6 fused to YFP and LSM8-HA in transgenic Arabidopsis. Total proteins were immunoprecipitated with anti-GFP antibody-coated paramagnetic beads from extracts of 7-day-old Arabidopsis seedlings. Proteins were detected with anti-GFP and anti-HA antibodies.