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. 2020 May 12;48(11):6280–6293. doi: 10.1093/nar/gkaa354

Figure 3.

Figure 3.

Characterization of novel pfd mutants. (A, B) The novel pfd2 mutant is a null allele. (A) Structure of the PFD2 gene indicating the position of the insertion. Boxes and line indicate exons and intron, respectively. Open boxes correspond to the 5′ and 3′ untranslated regions. The triangle indicates the Ds element insertion. Arrows indicate the position of the primers used for the semi-quantitative RT-PCR. Blue line represents 100 bp. (B) Semi-quantitative RT-PCR analysis of PFD2 expression in WT and mutant plants and the negative control (C-; water). The expression of UBQ10, a housekeeping gene, was used as control. (C) Rosette of 29-d-old plants of the indicated phenotypes at bolting. (D) Global morphological phenotype of 58-day-old plants of the indicated genotypes.