Skip to main content
. 2020 May 12;48(11):6280–6293. doi: 10.1093/nar/gkaa354

Figure 4.

Figure 4.

LSM8 protein level depends on PFD activity. (A, B) LSM8-GFP protein levels in the WT and pfd mutants grown at control temperature (20°C) or after being exposed to 4°C for one day. Representative blots are shown in (A). DET3 was used as loading control. (B) Plot showing LSM8-GFP/DET3 ratios. Data are average from three biological replicates. The statistical significance of the difference between mutants and WT is indicated by one, two, and three asterisks, which represent P < 0.01, 0.001 and 0.0001 in Bonferroni's multiple comparison test after an ANOVA test, respectively. (C) Gel filtration fractions were separated in 12% SDS-PAGE and LSM8-GFP detected by western blot with anti-GFP antibodies. (D) RT-qPCR analysis of the LSM8 and LSM8-GFP expression in standard conditions and 24 h after transfer to 4°C. Expression of PP2AA3 gene was used as reference for normalization. Data are average of three biological replicates. Error bars indicate the standard error of the mean. ns, no significant differences.