FIGURE 2.
Pemetrexed (PEM) sensitizes PC9 and A549 cells to activated T cells or natural killer (NK) cells. A and B, PC9 or A549 cells were cultured with PEM (2 µmol/L) for 2 d. Thereafter, untreated or PEM‐treated PC9 or A549 cells (5 × 104 cells) were cultured with activated T cells (1 × 105 cells) in 96‐well round plates for 6 h. After harvesting, whole cells were stained with anti–CD45‐APC, followed by annexin V‐FITC. A representative result from flow cytometry is shown. The numbers represent the percentages of annexin V+ cells. C, The results from three wells are shown. Similar results were obtained in two separate experiments. *P < 0.05. **P < 0.01. D and E, Similarly, untreated or PEM‐treated PC9 or A549 cells (5 × 104 cells) were cultured with purified NK cells (1 × 105 cells) for 12 h and analyzed by flow cytometry. A representative result from flow cytometry is shown. F, The results from three wells are shown. Similar results were obtained in two separate experiments. ** P < 0.01. ***P < 0.005