Table 3.

Immunohistochemical analysis of tumor immune microenvironment (TIME) in head and neck squamous cell carcinoma tissues

Case number	TIME classification	Immunohistochemistry			T‐cell infiltration (cell number)		PD‐1 expression on T‐cells		Treg infiltration
		PD‐L1	Galectin‐9	CEA CAM1	Stroma	Nest	Stroma	Nest	Cell number		Frequency in CD4+ T cells
									Stroma	Nest	Stroma	Nest
1	Excluded	–	Focal	–	++	+w	50%	90%	+	‐~+w	30%	70%
2	Excluded	–	–	–	+++	+w	80%	80%	+~++	–~+w	30%	nd
3	Excluded	>50%	30%	–	++	+w	50%	50%	+	–~+w	30%	10%
4	Excluded	>50%	Focal	30%	++	+w	50%	90%	+	–	50%	–
5	Excluded	–	–	Focal	+~++	+w	50%	90%	+w~+	–	nd	nd
6	Excluded	20%	–	Focal	+w~++	+w	nd	nd	+w~+	–~+w	nd	nd
7	Excluded	–	–	10%	++	‐	50%	nd	+	–	40%	nd
9	Excluded	5%	–	–	+~++	+w~+	30%	70%	+	+w	50%	50%
10	Desert	–	10%	–	‐~+w	‐	80%	nd	–~+w	–	50%	10%
11	Inflamed	>50%	>50%	>50%	++	++	90%	90%	+w~+	+w~+	20%	20%
12	Excluded	–	5%	–	+‐++	+w	nd	nd	+	–~+w	nd	nd
13	Excluded	>50%	–	–	+	+w~+	nd	nd	+w~+	+w	nd	nd
14	Excluded	<5%	20%	10%	+~++	‐~+w	30%	70%	+	–	60%	–
15	Excluded	>50%	30%	–	+w~++	+w~+	30%	70%	+	–~+w	60%	30%
17	Desert	–	–	–	+	+w	50%	90%	+	–~+w	50%	50%
18	Excluded	–	focal	–	+w~+	+w~+	50%	70%	+w	+w	nd	nd
20	Desert	–	–	–	‐~+	‐	50%	nd	+w	–	30%	–
21	Excluded	10%	focal	–	+~++	+w~+	50%	90%	+	+w	40%	50%
22	Excluded	>50%	30%	–	+++	‐	80%	nd	+~++	–	nd	nd
23	Excluded	–	10%	focal	+w~++	+w	50%	90%	+	–	nd	nd
24	Inflamed	>50%	>50%	–	+++	++	70%	90%	+~++	+	nd	nd
25	Inflamed	>50%	10%	–	+++	+~++	80%	80%	+~++	+	50%	50%
26	Excluded	30%	–	–	+	‐	50%	nd	+w	–	50%	–
27	Inflamed	>50%	>50%	10%	++~+++	+~++	80%	80%	+~++	+	60%	50%
28	Excluded	>50%	20%	–	++~+++	+w	30%	70%	+~++	–~+w	40%	40%

–, <10 cells/0.6 mm²; +w, 10 ~ 100 cells/0.6 mm²; +, 100 ~ 500 cells/mm²; ++, 500‐1000 cells/0.6 mm²; +++, >1000 cells/0.6 mm²; nd, not determined.