Figure 2.

Combined treatment with CH5126766 and R428 synergistically inhibits the growth of both HEY‐T30 and OVCAR‐5 cells. A, HEY‐T30 and OVCAR‐5 cells were treated with CH5126766 or R428 at indicated concentrations. 0 nM indicates the control treated with 0.1% DMSO. B, HEY‐T30 and OVCAR‐5 cells were treated with 0.1% DMSO or CH5126766 and/or R428 for 72 hours. The concentrations of agents applied to each cell line were as follows, and all the following experiments were carried out at the same concentrations: HEY‐T30, 200 nmol/L CH5126766 and/or 500 nmol/L R428; OVCAR‐5 200 nmol/L CH5126766 and/or 1000 nmol/L R428. CI, combination index. C, SKOV3 and OVCAR‐3 cells were treated with CH5126766 or R428 at indicated concentrations. 0 nM indicates the control treated with 0.1% DMSO. D, Evaluation of the combined treatment with 250 nmol/L CH5126766 and/or 500 nmol/L R428. Data represent the mean ± SD of 3 determinations. *P < .05, **P < .01 (vs. control). The cell growth rate of the 0.1% DMSO‐treated cells was regarded as 1. This experiment was carried out at least 3 times for each cell line. n.s., not significant