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Colony formation assay. HEY‐T30 and OVCAR‐5 cells were treated with 0.1% DMSO or CH5126766 and/or R428 at the indicated concentrations for 72 h. Cells were washed out and incubated with growth medium for 5 (HEY‐T30) or 7 (OVCAR‐5) additional days. Data on the colony area are presented as the percentage compared with the control (DMSO). This experiment was carried out at least 3 times for each cell line. *P < .05, **P < .01
