FIGURE 5.

ASH2L facilitates the recruitment of MLL1 to the cis‐regulatory elements of the ERα‐regulated gene in endometrial cancer cells. A, Schematic diagram of the searched estrogen response elements (EREs) at cis‐regulatory elements of PAX2. B, ChIP assay with indicated antibodies shows the recruitment of ASH2L and ERα at different EREs. C, ChIP assay shows the recruitment of indicated protein and histone modification at EREII/III. DNA fragments were amplified using qPCR with the primers indicated in (A). D and E, ChIP‐Seq profiles and the corresponding peaks of genomic regions around PAX2. Error bars represent mean ± SD (n = 3), *P < .05, **P < .01, ***P < .001