FIGURE 6.

ASH2L depletion inhibits the endometrial cancer cell growth/proliferation and migration. A‐F, Effect of ASH2L on cell growth/proliferation in Ishikawa cells. A, MTS assay was performed in Ishikawa cells carrying shCtrl or shASH2L (sequence from siASH2L #2). B and C, Colony formation experiments in Ishikawa cells with shCtrl and shASH2L. D and E, Promotion function of ectopic expression of ASH2L on cell proliferation was reduced by ERα depletion in Ishikawa cells. Scale bars, 100 μm. F, Expression of ASH2L and ERα in (D) was detected by western blotting. G‐J, Effect of ASH2L on cell migration in Ishikawa cells. oe‐ASH2L stands for overexpression of ASH2L. Data are shown as mean ± SD (n = 3), *P < .05, **P < .01, ***P < .001