FIGURE 2.

Inhibition of COX‐2 blocks epidermal growth factor (EGF)‐induced angiopoietin‐like 4 (ANGPTL4) expression and secretion. A, B, FaDu cells were treated with various concentrations of celecoxib, followed by 50 ng/mL EGF treatment for 6 h. Expression of ANGPTL4 mRNA and protein was analyzed using real‐time quantitative PCR and ELISA, respectively. C, shLacZ and shCOX‐2 (COX‐2 knockdown) cells were treated with 50 ng/mL EGF for 6 h. Expression of ANGPTL4 mRNA was analyzed using real‐time quantitative PCR. D, FaDu cells were transfected with 20 nmol/L siCOX‐2 (i), siANGPTL4 (ii), and scramble oligonucleotides by lipofection and then treated with 50 ng/mL EGF for 6 h. Real‐time quantitative PCR was carried out for analysis of mRNA levels of ANGPTL4 and COX‐2. Values represent mean ± SEM of 3 replicate analyses