FIGURE 3.

Cyclooxygenase‐2‐derived prostaglandin E₂ (PGE₂) significantly induces angiopoietin‐like 4 (ANGPTL4) expression. A‐C, Cell lines (A) and FaDu cells (B, C) were treated with 20 μmol/L PGE₂ for 6 h or the indicated period of time, or with various concentrations as indicated. Expression of ANGPTL4 was analyzed using real‐time quantitative PCR. D, E, Cells were transfected with 20 nmol/L COX‐2 siRNA (siCOX‐2) and scramble oligonucleotides by lipofection and then treated with 20 μmol/L PGE₂ and 50 ng/mL epidermal growth factor (EGF) or 20 μmol/L celecoxib for 6 h. mRNA levels of COX‐2, ANGPTL4, and GAPDH were analyzed by RT‐PCR and examined by 2% agarose gel electrophoresis or real‐time quantitative PCR. Values represent mean ± SEM of 3 replicate analyses