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. 2020 Apr 30;111(6):2041–2051. doi: 10.1111/cas.14399

FIGURE 2.

FIGURE 2

Ribosomal protein S6 (RPS6) knock‐down suppressed the sphere‐forming potential and expression of glioblastoma multiforme (GBM) stem cell (GSC) markers in glioma cells. A, GBM cells (U251MG) were transfected with control siRNA (siCont) or siRNA specific for RPS6 (siRPS6). RPS6 expression was confirmed at the protein level upon western blotting. B, RPS6 knock‐down experiments; sphere‐forming potential in GBM cell line (U251MG) cultured in 10% FCS and NSC medium. Graph showing the number of spheres (>50 µm). C, Western blot data showing that RPS6 knock‐down affected the expression of GSC markers Nestin and SOX2 in GBM cell line (U251MG) cultured in neural stem cell (NSC) medium. A graph showing the expression of Nestin and SOX2 in siRPS6‐transfected cells or siCont‐transfected cells. D, Empty control vector (Control) or RPS6‐expressing plasmid vector (OE‐RPS6) was transfected into GBM cell line (U251MG). Images displaying sphere formation by OE‐RPS6. Graph showing the number of spheres (>50 µm) in NSC medium (values are presented as means ± SD of triplicate samples, *P < .05 siCont vs siRPS6 and Cont vs OE‐RPS6). Scale bar, 100 µm