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. 2020 Apr 30;111(6):2041–2051. doi: 10.1111/cas.14399

FIGURE 3.

FIGURE 3

Ribosomal protein S6 (RPS6) regulates stemness in glioblastoma multiforme (GBM) cells via STAT3 signaling. A, Western blot showing the expression of pSTAT3 and STAT3 in GBM cell line (U251MG) transfected with siRPS6 or siCont in neural stem cell (NSC) medium. Graph showing relative protein expression of pSTAT3/STAT3 (B) Treatment with 10 and 25 µmol/L of AG490 (JAK‐STAT inhibitor) affected the sphere‐forming potential of GBM cell line (U251MG) relative to the control. C, Images and graphs showing the sphere‐forming potential of GBM cell line (U251MG) and the number of spheres (>50 µm). Values are presented as mean ± SD of triplicate samples; *P < .05, **P < .01. Scale bar, 500 µm