FIGURE 6.

MicroRNA (miR)‐93 suppressed liver metastasis in vivo. A, Schematic representation of the splenic injection of tumor cells and competitive transplantation assays. For the competitive transplantation assay, MDA‐MB‐231‐ZsGreen cells overexpressing miR‐93 (miR‐93 ZsGreen) and MDA‐MB‐231 mCherry competitor (mixed 1:1) were transplanted into the spleen of immunodeficient NSG mice. B, Gross examination of the development of metastases in the liver 21 d after intrasplenic injection of MDA‐MB‐231 cells stably infected with miR‐93‐expression or control lentivirus. Number of tumors on the surfaces of the liver was counted. Arrows indicate representative liver metastases. Data are presented as mean ± SD. n = 6, *P < .05. Scale bar = 10 mm. C, Donor chimerism of the cancer cells metastasized to the liver was evaluated by FACS. The liver was perfused, dissociated, and analyzed 3 wk after transplantation. Percentage of donor chimerism of the cancer cells metastasized in the liver after competitive transplantation assay. Data are presented as mean ± SD. n = 3, *P < .05