Figure 3. Peptide 2 drastically reduces the rate of Aβ₄₂ fibril elongation.

(A) The change in ThT fluorescence during the elongation phase of Aβ₄₂ (100 µM) fibril formation in the absence (black) and presence (grey, blue and purple) of inhibitors at a range of molar ratios (0–2.5 h from Figure 2A). (B) The rate of Aβ₄₂ fibril elongation as a function of inhibitor concentration (mM) was fitted with a non-linear one-phase decay fit. (C) ThT fluorescence assay of Aβ₄₂ (10 µM) spiked after 4.5 h (red dashed line) with peptides 1 and 2 (100 µM) during incubation where no decrease in fibrilization was observed. Data reported is presented as mean ± SEM (n = 3).