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. 2020 Jun 13;209(4):447–459. doi: 10.1007/s00430-020-00683-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Overlap between actin and OBSL1. HaCaT cells were transfected with V5-OBSL1 and 1 day post transfection cells were incubated with PsVs for 3 h, fixed and stained for V5 with an antibody and for actin with phalloidin conjugated to a fluorophore. The linear lookup tables illustrate the channels for V5-OBSL1 and actin in green and magenta, respectively. The similarity between the OBSL1 and actin signals was quantified by calculation of the Pearson Correlation Coefficient (PCC). Values are shown as means ± SD (n = 21–27 ROIs, shown in Fig. S3, collected from 39 cells pooled from 4 biological replicates). ***, p < 0.001 (unpaired Student’s t-test, comparing aggregated to non-aggregated actin)