Fig. 6.

Morphology of endocytic organelles. a HepG2 cells were transfected with V5-OBSL1. One day post transfection, cells were incubated with PsVs for 3 h and then exposed to a brief ultrasound pulse, which removes the upper parts of the cells leaving behind the basal cell membranes. These membrane sheets were fixed and stained for V5 and PsVs with antibodies, and for actin with phalloidin conjugated to a fluorophore. The linear lookup tables illustrate the channels for PsV, V5-OBSL1 and actin in red, green and magenta, respectively. Images were screened for locations at which PsVs are close to actin and OBSL1 positive structures. Shown is the largest example we found. b Electron micrographs of HeLa cells incubated with HPV16 PsVs (visible as black dots with a size of 50–60 nm). The lower image shows a roughly 800 nm long tubular and virus filled endocytic vesicle. Filamentous actin (see arrows) is in close proximity to the virus containing organelles. Images are

taken from an experiment previously described [15]