Figure 2.

In Vitro Validation of Radiotracer Uptake by cNIS⁺/GFP⁺ Canine Myoblasts

(A) Measurement of ^{99 m}TcO⁻ uptake efficiency by canine myoblasts sorted for GFP expression. The experiment was performed with cells under proliferation or after 5 days of differentiation. Sodium perchlorate (NaClO₄) was used for specific inhibition of NIS activity. NIS⁻ myoblasts were used as negative control. Values were normalized to 1 × 10⁵ cells. *p < 0.05 and ****p < 0.0001 of 3 independent experiments. (B) Imaging of in vitro cells. Different concentrations of cNIS myoblasts, 1 × 10⁶, 0.5 × 10⁶, 10⁵, and twice 0.5 × 10⁵ imaged after incubation with 185 kBq of ^{99 m}TcO4⁻ imaged in a 12-well plate (upper right). Graphs represent ratio of cell uptake/surface area normalized by the background noise. Representative graph; each column represents three replicates. Statistical analysis performed with GraphPrism using one-way ANOVA test. *p < 0.05.