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. 2020 Jun 12;11:2979. doi: 10.1038/s41467-020-16794-5
Plasmid Name Vector Restriction Site Forward (F) and Reverse primers (R)
EGFP-CA-Rab11 Addgene#56444 Site-directed mutagenesis F – CAGCAGGGCTAGAGCGATATCGAGCTATAACATCAGCATATTATC R - GCTCGATATCGCTCTAGCCCTGCTGTGTCCCATATCTGTG
Atg5 siRNA pSuper BglII/HindIII F – GGAAGATCTGGCTCACTTTATGTCATGTAAGCTTGGG R -CCCAAGCTTACATGACATAAAGTGAGCCAGATCTTCC
Scrambled oligonucleotide pSuper BglII/HindIII F – GGAAGATCTGACGTGAACGGATAACACTAAGCTTGGG R -CCCAAGCTTAGTGTTATCCGTTCACGTCAGATCTTCC
HA-Atg5 pGW1 XmaI/KpnI F – TCCCCCCGGGATGTGTGGTTTGGACGAATTCCAACTTGTT R - CGGGGTACCTGAGCAGCGTAATCTGGAACGTCATATGGAT
siRNA-resistant Atg5 Atg5-siRNA BglII/HindIII F – GGAAGATCTCGCTCATTTCATGTCATCTAAGCTTGGG R - CCCAAGCTTCCCAAGCTTAGATGACATGAAATGAGCG
HA-Beclin-1 pGW1 BglII/EcoRI F – CGCGGATCCATGGAAGGGTCTAAGACGTCCAACAACAG R - CCGGAATTCAGCGTAATCTGGAACATCGTATGGGTAGCGG
G2CT Addgene, #66709 EcoRI/Kpnl F- CCGGAATTCAAGCGGATGAAGCTGAACATCAACCCTAGC R – CGGGGTACCGCTAGGGTTGATGTTCAGCTTCATCCGCTT
Scrambled peptide Addgene, #66709 EcoRI/Kpnl F- CCGGAATTCCGGAAGAACAGCAAGATGCTGATCCCT R – CGGGGTACCAGGGATCAGCATCTTGCTGTTCTTCCG
mRFP-LC3 pGW1 KpnI/EcoRI F – CGGGGTACCTCATGGCCTCCTCCGAGGACGTCATC R - GCGCGAATTCTCACAAGCATGGCTCTC
mRFP-LC3BG120A pGW1-mRFP-LC3 Site-directed mutagenesis F – CCCAGGAGACGTTCGCGACAGCACTGGCTGTTACATAC R - CCAGTGCTGTCGCGAACGTCTCCTGGGAGGCATAGACC