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. 2020 Jun 12;10:9540. doi: 10.1038/s41598-020-66374-2

Figure 2.

Figure 2

Genome-wide distribution of TuMV-derived siRNAs in wild type Arabidopsis thaliana and de-novo assembly of the TuMV genome. 21- to 24-nt virus-derived siRNAs were pooled and correspond to samples in Fig. 1. Abundance was normalized to reads per million. (a) Genome-wide distribution of TuMV-derived siRNAs by match class in inoculated rosette leaves and in systemically infected inflorescence. (b) Abundance of siRNAs with one or two mismatches relative to siRNAs with no mutations. (c) Relationship between siRNAs of positive and negative polarity and containing mutations. (d) De-novo assembly of the TuMV genome using virus-derived siRNAs without mutations. Lines represent contigs and red arrowheads point to gaps in the assembly. In this TuMV clone, HC-Pro is marked with 6xHIS at the N terminus27.