Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 12;10:9540. doi: 10.1038/s41598-020-66374-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Genome-wide distribution of siRNAs derived from suppressor-deficient TuMV-AS9 and de-novo genome assembly. siRNAs 21- to 24-nt were pooled and classified based on the number of mismatches. Numbers represent average and standard error from two biological replicates from inoculated rosette leaves (7 dpi) and systemically infected cauline leaves (15 dpi) of ago2-1 single mutant Arabidopsis. Number of reads were normalized to reads per million. (a) Unique sequences and their abundance by polarity. The ratio of siRNAs with mismatches to siRNAs with no mutations is indicated for each tissue. (b) Genome-wide distribution of virus-derived siRNAs by match class, polarity, and tissue. (c) Correlation between siRNAs of positive and negative polarity, by match class and tissue. (d) De-novo assembly of the TuMV-AS9 genome using siRNAs with no mutations. Coverage is expressed as percent of the genome. Red arrow heads point to gaps in the assembly.