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. 2020 Jun 12;10:9540. doi: 10.1038/s41598-020-66374-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of suppressor-deficient TuMV-AS9-derived siRNAs in inoculated rosette leaves and systemically infected cauline leaves of ago2-1 single mutant Arabidopsis. 21- to 24-nt siRNAs were pooled and correspond to samples in Fig. 5. Values are the average of two biological replicates. Unique sequences were counted per tissue and their abundance normalized to reads per million. Virus-derived siRNAs with (a) no mutations and (b) with one or two mismatches. (c) Accumulation of single nucleotide polymorphisms (SNPs) in virus-derived siRNAs in inoculated rosette leaves, in systemically infected inflorescence, and their correlation. The asterisk (*) indicates significant difference (p-value < 0.001). (d) Genome-wide distribution of single nucleotide polymorphisms in virus-derived siRNAs estimated in a 50-nt window.