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. 2020 Jun 12;10:9540. doi: 10.1038/s41598-020-66374-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome-wide distribution of Wheat streak mosaic virus (WSMV)-derived siRNAs and de-novo genome assembly. SiRNAs (21- to 24-nt) were pooled and correspond to two contrasting cultivars, Arapahoe (susceptible) at 18 °C and 27 °C and Mace (susceptible at high temperature) at 27 °C. Abundance was normalized to reads per million. (a) Genome-wide distribution of WSMV-derived siRNAs in cultivar Arapahoe, and (b) cultivar Mace. (c) De-novo assembly of the WSMV genome using siRNAs with no mutations. Genome coverage is indicated in percent. Red arrow heads point to gaps (12 nt) in assembly, which map to the coat protein from nt 8331 to 8343.