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. 2020 Jun 12;11(6):454. doi: 10.1038/s41419-020-2597-7

Fig. 5. G-Rg3 inhibited Rapamycin-induced autophagy flux in vitro.

Fig. 5

a Autophagy was triggered by Rapamycin (Ra) at 100 or 200 nM in HSC-T6 cells with G-Rg3 pretreatment, and autophagy-related proteins were detected by western blot. b Immunofluorescence staining of p62 was conducted in HSC-T6 cells. G-Rg3 pretreatment with 16 μM and Ra were conducted with 100 or 200 nM. Relative fluorescence intensity was quantified. Red arrows indicated p62-positive cytoplasm expression and its location in HSC-T6 cells. Values were shown as the mean ± S.D; *p < 0.05, **p < 0.01 versus Normal group; #p < 0.05, ##p < 0.01 versus Ra group, respectively.