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. 2020 Jun 12;11:2991. doi: 10.1038/s41467-020-16729-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The active sites of SxtT (purple) and GxtA (gray) are relatively similar to one another except for the replacement of SxtT residues Met255 and Thr276 with GxtA residues Tyr255 and Val276. b Reactions of SxtT wild-type and variants with STX (2). c Reactions of GxtA wild-type and variants with β-STOH (1) and the mixtures of products generated in these reactions. Data are presented as mean values ± SD. Reaction conditions: 5 µM SxtT/GxtA, 5 µM VanB, 200 µM β-STOH (1), 500 µM NADH, incubated at 30 °C for 2 h.