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. 2020 Jun 12;11:2978. doi: 10.1038/s41467-020-16789-2

Fig. 3. ARS2 directly activates transcription of MGLL.

Fig. 3

a Heatmap analysis of gene expression in X01 cells infected with a shARS2-expressing lentiviral or shCtrl construct. b, c PCR (left) and immunoblot (IB) analysis (right) of ARS2 and MAGL in GSCs (578 and 0502 cells) infected with an ARS2-expressing lentiviral or vector construct. GAPDH or α-tubulin was used as a loading control. d, e qRT-PCR (left) and IB analysis (right) of ARS2 and MAGL in GSCs (528 and X01 cells) infected with a shARS2-expressing lentiviral or shCtrl construct. Vinculin was used as a loading control. All error bars represent mean ± SEM (n = 3). *P < 0.05, **P < 0.01, t-test. f, g Immunoprecipitated chromatin was analyzed by qPCR using 3- and 4-specific primers on the promoter region of the MAGL gene. All error bars represent mean ± SEM (n = 3). *P < 0.05, **P < 0.01, t-test. h Luciferase reporter assays of MAGL activity in GSCs (X01 cells) infected with a shARS2–expressing lentiviral or shCtrl construct. All error bars represent mean ± SEM (n = 3). **P < 0.01, t-test.