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. 2020 Jun 12;11:2983. doi: 10.1038/s41467-020-16690-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Real-time metabolic heat output profiles of referential C. elegans from L1 stage to Adult (Day 2) stage. b Metabolic heat output measurements of N2 wild-type worms (n = 61 independent trials) at different stages from L1 to Adult (Day 4). The red and blue triangles represent the AMR and SMR of individual worms, respectively, and are connected by a black dashed line to identify measurements from individual worms. The inset shows the mass-specific AMR (red) and SMR (blue) averaged over 61 N2 wild-type worms while the error bars represent the standard deviation (±SD) about the mean. c SMR vs. body size on a log-log plot to extract an allotropic scaling constant of 0.76 from (n = 52 independent trials) wild-type N2 samples from L3 to Adult (Day 3). The purple solid line represents the best-fit with R-squared error of 0.75. The purple shaded region indicates the 95% confidence interval.