Figure 4.

DNA fragmentation in cells exposed to D4. Jurkat cells were cultured for 2 or 4 hours in the presence of 10 μg/ml anisomycin (An), 0.15% H₂O₂, 1% D4 or 0.3% D4. Cell lysates were depleted of high-molecular-weight DNA-containing complexes and relatively small fragmented DNA was fractionated by electrophoresis in a 1.5% agarose gel. Note that this figure was composed with different (cropped) parts of the same gel, indicated by the dividing lines, as further illustrated in the Supplementary Information.