Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 12;10:9558. doi: 10.1038/s41598-020-66666-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cell death induction by D4 in HeLa and HEp-2 cell lines. HeLa (A, B) and HEp-2 (C, D) cells were cultured for the indicated time periods in the presence of 1% D4, or for 4 hours in the presence of 10 μg/ml anisomycin (An) or 0.15% H₂O₂. Cell lysates were analyzed by western blotting using patient sera reactive with U1-70K (A,C) or Topo I (B,D). The apoptotic cleavage products of U1-70K and Topo I (arrows) are indicated with arrowheads; the necrotic cleavage product of Topo I is indicated with an asterisk. As a reference, material from anisomycin- and H₂O₂-treated Jurkat cells (Jk) was analyzed in parallel (lanes 1-2). The positions of molecular weight markers are indicated on the left of each panel. Note that in all panels a cropped part of the respective blot is shown, as further illustrated in the Supplementary Information.