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. 2020 Jun 12;11(6):453. doi: 10.1038/s41419-020-2665-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The apoptotic rate of NHKs in response to Poly(I:C), detected by flow cytometry assay. b, c The RNA levels (b) and protein levels (c) of IFIH1 in NHKs with Poly(I:C) concentration rose, detected by qRT-PCR assay and Western-blot respectively. d The expression of MDA5 in NHKs in response to 0.6 μg/ml Poly(I:C) within 48 h, determined by Western-blot assay. e The expression of MDA5 in NHKs stimulated by 0.6 μg Poly(I:C) for 24 h, detected by immunofluorescence assay. Bar = 10 μm. The data are representative of three independently performed experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. ns, not significant.