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. 2020 Jun 12;11(6):453. doi: 10.1038/s41419-020-2665-z

Fig. 4. The secretion of CXCL10 and CXCL16 is orchestrated by the combination of MDA5 and MAVS.

Fig. 4

a The Western-blot of MAVS in NHKs in response to Poly(I:C). b The formation of MAVS aggregates with the treatment of Poly(I:C) for 18 h in NHKs, or interfering MDA5 by using MDA5 siRNA (si-MDA5) prior to 18 h Poly(I:C) treatment in HaCaT cells, detected by SDD-AGE assay. c The colocalization of MDA5 and MAVS in NHKs and HaCaT cells, detected by immunofluorescence assay. Bar = 10 μM. d, e The mRNA and secretion levels of CXCL10 and CXCL16 when interfering MDA5 (d) or interfering MAVS by using MAVS siRNA (si-MAVS) (e) prior to Poly(I:C) treatment in HaCaT cells. The data are presented as the mean ± SD across three independently performed experiments. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001. ns, not significant.