Skip to main content
. 2020 Jun 12;11(6):453. doi: 10.1038/s41419-020-2665-z

Fig. 5. NF-κB and IRF3 is decisive for secretion of CXCL10 and CXCL16.

Fig. 5

a The Western-blots of total P-65, phosphor-P65, total IRF3 and phosphor-IRF3 in NHKs in the indicated time in response to Poly(I:C). b, c The Western-blots of total P-65, phosphor-P65, total IRF3 and phosphor-IRF3 when interfering MDA5 (si-MDA5) (b) or interfering MAVS (si-MAVS) (c) in HaCaT cells prior to 24 h 0.6 μg/ml Poly(I:C) treatment. d, e The mRNA and secretion levels of CXCL10 and CXCL16 when interfering NF-κB by using NF-κB siRNA (si-NF-κB) (d) or interfering IRF3 by using IRF3 siRNA (si-IRF3) (e) for 24 h prior to 0.6 μg/ml Poly(I:C) stimulation for 24 h in HaCaT cells. The data are presented as the mean ± SD across three independently performed experiments. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001. ns, not significant.