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. 2020 Jun 12;3:304. doi: 10.1038/s42003-020-1024-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Dots represent a single trial for groups of 6, 12 and 24 flies at low (blue), medium (orange) and high (grey) densities. The mean for each group size-density treatment is indicated by a horizontal line. Letters indicate statistical significance (α = 0.008) after outlier removal (Supplementary Fig. 3). a Movement. Movement does not change across different group sizes and densities. Density: F_(2,183) = 0.96, p = 0.386; Group size: F_(2,183) = 0.02, p = 0.982; Density × Group size: F_(4,183) = 1.52, p = 0.198. Group size 6: low—n = 20, medium—n = 21, high—n = 23; Group size 12: low—n = 22, medium—n = 20, high—n = 21; Group size 24: low—n = 23, medium—n = 19, high—n = 23. b Interaction rate. Interaction rates increase with increasing density and group size. Density: F_(2,184) = 78.27, p < 0.008; Group size: F_(2,184) = 538.27, p < 0.008; Density × Group size: F_(4,184) = 9.39, p < 0.008. Group size 6: low—n = 20, medium—n = 21, high—n = 24; Group size 12: low—n = 22, medium—n = 20, high—n = 22; Group size 24: low n = 22, medium = 19, high = 23. c Clustering coefficient. Flies in groups of 6 and 12 have lower clustering coefficient than groups of 24 flies. There is no effect of density on clustering coefficient. Density: F_(2,176) = 2.70, p = 0.070; Group size: F_(2,176) = 47.50, p < 0.008; Density × Group size: F_(4,176) = 1.10, p = 0.360. Group size 6: low n = 20, medium = 21, high = 21; Group size 12: low n = 21, medium = 20, high = 22; Group size 24: low n = 22, medium = 17, high = 21. d Betweeness centrality. Flies in groups of 6 have lower betweeness centrality than groups of 12 and 24 flies. Density: F_(2,178) = 6.03, p < 0.008; Group size: F_(2,178) = 809.95, p < 0.008; Density × Group size: F_(4,178) = 1.76, p = 0.140. Group size 6: low n = 20, medium = 21, high = 23; Group size 12: low n = 20, medium = 19, high = 22; Group size 24: low n = 21, medium = 18, high = 23.