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Determination of the fluorescent detection range. Fluorescent images of (A) 4 µM PKH only control, (B) 4 µM PKH-labelled EVs (1.5 µg/mL), (C) 0.16 µM PKH only control,(D) 0.16 µM PKH-labelled EVs (0.3 µg/mL) in diluent C. (E) Representative line scan analysis of fluorescent images (A–D). Inset shows the line scan analysis of background, 0.16 µM PKH only control and 0.16 µM PKH-labelled EVs (0.3 µg/mL) in diluent C.
