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. 2020 Jun 12;10:9533. doi: 10.1038/s41598-020-66434-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Size characterization of CFSE labelling of EVs by Nanoparticle Tracking Analysis (NTA). Fluorescent images of (A) 16 4 µM CFSE only control (B) 4 µM CFSE-labelled EVs (0.3 µg/mL) in PBS. (C) Representative line scan analysis of fluorescent images (A,B). (D) Size distribution of EVs only control, CFSE only control, and CFSE-labelled EVs in PBS (n = 7). (E) Size mode of EVs only control, CFSE only control, and CFSE-labelled EVs (error bars represent 95% confidence interval of the mean) in PBS n = 7, p value < 0.05).