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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: J Immunol. 2020 Apr 24;204(12):3262–3272. doi: 10.4049/jimmunol.2000030

Figure 1. Peritoneal B-1a cells have higher Bmi1 dependency than other lymphoid subsets.

Figure 1.

A. Bmi1 mRNA expression measured by qPCR in various sorted populations from adult BM, peritoneal cells (PerC), or spleen (Sp). KLS: kit+Sca-1+lin− HSC population, FO: follicular B-cells, MZ: marginal zone B-cells. B. Bmi1 expressions in various fetal liver (FL) progenitor populations. CLP: common lymphoid progenitors. C. Marked reduction of peritoneal cell count in Bmi1−/− mice (n=6, p<0.001). D. The frequency of IgM+ B-cell and CD5+ T-cells in the WT and Bmi1−/− peritoneal cavity is depicted (n=5, ** p<0.01). E. Representative FACS plots for peritoneal B-1a cells in WT and Bmi1−/− mice are depicted. F. The frequency of B-cell subsets among the peritoneal IgM+ cells is depicted. The percentage of B-1a cells is reduced (n=5, ***p<0.001). (G-J) Total cell number of FLs (G), representative FACS plots for lin−AA4.1+CD19+B220− B-1 progenitor population in the FL (H), the frequency (I) and absolute number (J) of FL B-1 and B-2 progenitor cells in WT and Bmi1−/− embryos are depicted. B-1 progenitor cell number is increased in the Bmi1−/− FL (n=6, *p<0.03). White bar & circle: WT, black bar & circle: Bmi1−/−. All data were obtained from experiments more than 3 times.