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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: J Immunol. 2020 Apr 24;204(12):3262–3272. doi: 10.4049/jimmunol.2000030

Figure 3. B-cell-specific deletion of Bmi1 leads to reduction in the B-1a cell pool and loss of self-renewal ability.

Figure 3.

The efficiency of CD19-Cre recombinase was confirmed by genomic PCR (A) in FACS-sorted B-1 cells or bead-selected spleen B-cells (B). C: Total peritoneal cell numbers in WT, CD19Cre/+, CD19Cre/+: Bmi1F/+, and CD19Cre/+: Bmi1F/F mice are shown. No obvious difference was found among the 4 groups. D: The numbers of peritoneal B-1a cells from 4 groups at different ages are shown. (n=7-12 for each genotype for each age point, *p<0.05, **p<0.01). E: The number of B-1a cells in CD19Cre/+: Bmi1F/+ and CD19Cre/+: Bmi1F/F mice at 3-9 months of age in the same litter is depicted. The line connects the mice in the same litter. The frequency of lymphoid subsets in the peritoneal cavities (F) and the spleens (G, H) of WT, CD19Cre/+, CD19Cre/+: Bmi1F/+, and CD19Cre/+: Bmi1F/F mice are shown (n=6). I, J: Competitive peritoneal cell transplantation assays. The FACS plots of peritoneal B-1 cells of competitive peritoneal cell-transplanted mice (I) and the donor percentage (J) are shown. K, L: Fetal liver MNC transplantation assays. The percentage of donor-derived lymphoid subsets in the peritoneal cavities of transplanted mice with WT or CD19Cre/+: Bmi1F/F FL MNCs at 6 weeks (K) and 9 months (J) post-transplant (n=3 for each genotyping, at each time point). M: B-1a cell proliferation upon stimulation with TRL7 agonist. Cell number was counted 48 h after stimulation (n=3). MZ: marginal zone B cell, FO: follicular B cell, CD5T: CD5+ T cell. All data were obtained from experiments more than 3 times.