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. 2020 Jun 9;13:5283–5291. doi: 10.2147/OTT.S248457

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© 2020 Liu et al.

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Interaction between LncRNA PTTG3P and microRNA-155-5P. (A) Bioinformatics and luciferase reporter gene experiments was applied to detect the binding of LncRNA PTTG3P and microRNA-155-5P; (B) qRT-PCR was performed to verify the expression level of microRNA-155-5P after knockdown of LncRNA PTTG3P in CRC cell lines HCT-8 and HCT-116; (C) qRT-PCR was performed to detect the level of microRNA-155-5P expression in CRC tumor tissues and non-tumor tissues; (D) Kaplan Meier survival curves was plotted to evaluate the relationship between microRNA-155-5P expression and prognosis; (E) qRT-PCR was performed to detect the correlation between LncRNA PTTG3P and microRNA-155-5P expression levels; (F) qRT-PCR was performed to detect the level of LncRNA PTTG3P after microRNA-155-5P was knocked down in CRC cell lines HCT-8 and HCT-116. Relative expression was demonstrated by qRT-PCR, and GAPDH, U6 was used as a housekeeping gene and expression determined using 2^–∆∆CT method. Data are average ± SD, *P <0.05, **P <0.01, ***P <0.001.