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. 2020 Jun 9;12:4347–4356. doi: 10.2147/CMAR.S250670

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© 2020 Lu et al.

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DSCAM-AS1 acted as a molecular sponge for miR-204 in CC cells. (A) miR-204 contains the potential binding site for DSCAM-AS1 predicted by Starbase2.0. (B) The luciferase activity was detected in CC cells after co-transfected with wild type (WT) or mutant (Mut) DSCAM-AS1 reporter and miR-204 mimics or miR-NC. (C) The relative DSCAM-AS1 expression levels in CC cells following miR-204 mimics transfection. (D) The relative miR-204 expression levels in CC cells after DSCAM-AS1 knockdown. (E) The relative expression of miR-204 in colon cancer tissues and adjacent normal tissues detected by qRT-PCR. (F) miR-204 expression in 4 CC cell lines and the normal colon epithelial cell line NCM460 determined by qRT-PCR. (G) The linear relationship between DSCAM-AS1 and miR-204 expression levels in CC tissue samples. Data were presented as mean ± SD. *P < 0.05.