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. Author manuscript; available in PMC: 2020 Sep 24.
Published in final edited form as: ACS Nano. 2019 Sep 10;13(9):10161–10178. doi: 10.1021/acsnano.9b03334

Figure 3. Compositions of the FNC-assembled pDNA/lPEI nanoparticles.

Figure 3.

(A) The fraction of bound lPEI and the composition of the assembled nanoparticles remained similar when nanoparticles were prepared at different input pDNA concentrations or with different plasmids; (B) Bound vs. free lPEI amount and proportions with an input N/P ratio from 3 to 6 for gWiz-Luc and gWiz-GFP nanoparticle formulations assembled under a turbulent mixing condition (Q = 20 mL/min, τM = 15 ms < τA) and a laminar mixing condition (Q = 5 mL/min, τM= 790 ms > τA). Labels: Luc and GFP for gWiz-Luc and gWiz-GFP plasmid nanoparticles, respectively; (C) Bound lPEI fraction and zeta-potential of nanoparticles prepared with 50 or 200 μg/mL of gWiz-Luc pDNA under different flow rates, suggesting that all gWiz-Luc/lPEI nanoparticles share the same average composition; (D) A representative Zimm plot for I2/lPEI nanoparticles with a molar mass of 5.32 × 107 Da, also showing the second viral coefficient A approaching zero; (E) Representative Debye plots for gWiz-GFP/lPEI nanoparticles prepared by varying input pDNA concentration for I2 plasmid; (F, G) Calculated weight average pDNA copy numbers per nanoparticle (N) for preparations from lPEI and I2 or gWiz-GFP plasmids at N/P = 4 (F) or gWiz-Luc plasmid at different concentrations and N/P ratios (G).