. Author manuscript; available in PMC: 2020 Sep 24.

Published in final edited form as: ACS Nano. 2019 Sep 10;13(9):10161–10178. doi: 10.1021/acsnano.9b03334

Table 1.

pDNA/lPEI nanoparticles prepared with different mixing conditions and N/P ratios

Nanoparticle Code	Plasmid	Q (mL/min)	Input N/P	Z-average Diameter^* D_z (nm)	Weight Average $\bar{N}$
W1	gWiz-Luc	20	3	88.2 ± 30.0	9.1
W2			4	81.2 ± 25.5	6.1
W3			5	79.3 ± 40.4	5.0
W4			6	77.5 ± 30.1	4.4
W5		5	3	153.0 ± 61.9	40.7^**
W6			4	158.9 ± 64.5	45.6^**
W7			5	148.1 ± 62.8	37.0^**
W8			6	155.5 ± 72.0	42.8^**
W9		Pipetting	6	171.2 ± 86.3	57.0^**
P1	PEG-fLuc	20	3	81.4 ± 21.2	8.1^**
P2			4	86.0 ± 25.1	9.5^**
P3			5	82.7 ± 28.4	8.5^**
P4			6	81.0 ± 26.6	8.0^**
P5		5	3	146.9 ± 57.8	45.7^**
P6			4	151.1 ± 60.2	49.7^**
P7			5	145.6 ± 54.7	44.5^**
P8			6	134.1 ± 46.6	34.9^**

Reported as Z-average hydrodynamic diameter ± DLS size standard deviation.

^**

Calculated based on Eqs. 5 and 14.